AFFINITY CHROMATOGRAPHY : Principles and Methods

Affinity Chromatography is a highly selective technique used for the purification of biomolecules, such as proteins, enzymes, and antibodies, based on specific binding interactions between the target molecule and a ligand attached to the stationary phase. The stationary phase contains ligands that are specific to the molecule of interest, allowing it to bind selectively while other components are washed away. The bound molecule is then eluted by altering the conditions (such as pH, ionic strength, or adding a competitive ligand) to disrupt the interaction. This method provides high purity and yield, making it invaluable in biochemical research and pharmaceutical manufacturing for isolating proteins, nucleic acids, and other biomolecules with high specificity.